Affinity PurifiedAcid-sensing ion channel 2 Antibody
Verified Applications
ELISA, IP, WB
Host
Rabbit
Species Cross Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide taken within amino acid region 450-512 on human Acid-sensing ion channel 2 protein.
Physical Properties
Quantity
100 µg
Volume
200 µl
Concentration
0.5-1.5 µg/µl in antibody stabilization buffer
Immunoglobulin
IgG
Form
Affinity Purified
Purification Method
Immobilized antigen affinity chromatography
Clonality
Polyclonal
Storage
-20⁰C for long term storage
Application Protocol
ELISA
1:50,000
Immunoprecipitation
1:200
Western Blot
1:2,500-1:5,000
Protein
Uniprot #
Q16515
Overview
Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li+ and K+. Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.
Molecular Function
acid-sensing ion channel activity; ion gated channel activity; ligand-gated sodium channel activity
Subcellular Location
Cell membrane; Multi-pass membrane protein
Expression
Brain and spinal cord. Isoform 1 is also detected in testis, liver, colon and ovary.
Structure
Homotrimer or heterotrimer with other ASIC proteins.