General Information

Product

IRF8 Antibody

Verified Applications

ELISA, IHC, IP, WB

Host

Rabbit

Species Cross Reactivity

Mouse, Rat

Immunogen

Synthetic peptide taken within amino acid region 350-400 on mouse Interferon Regulatory Factor 8 protein.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

P23611

Overview

Plays a role as a transcriptional activator or repressor. Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5"-TGAnTCA/GAAA-3"), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (PubMed:22992524).

Molecular Function

DNA binding

DNA binding transcription factor activity

RNA polymerase II proximal promoter sequence-specific DNA binding

RNA polymerase II regulatory region sequence-specific DNA binding

transcriptional repressor activity, RNA polymerase II proximal promoter sequence-specific DNA binding

Subcellular Location

Nucleus, cytoplasm

Expression

Predominantly in lymphoid tissues.

Structure

Belongs to the IRF family.

Alternative Nomenclature

H ICSBP antibody

HICSBP antibody

ICSBP 1 antibody

Interferon consensus sequence binding protein 1 antibody

Interferon regulatory factor 8 antibody

IRF 8 antibody

MYLS antibody