General Information

Product

Filamin B (phospho Ser 2478) Antibody

Description

Affinity Purified Phospho-Filamin-B Antibody N-epitope

Verified Applications

ELISA, IP, WB

Host

Rabbit

Species Cross Reactivity

Human, Mouse

Immunogen

Synthetic peptide taken within amino acid region 2450-2500 on human Phospho-Filamin-B. Phosphorylated 2478 serine residue.

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.5-1.5 µg/µl in antibody stabilization buffer

Immunoglobulin

IgG

Form

Affinity Purified

Purification Method

Immobilized antigen affinity chromatography

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰C for long term storage

Application Protocol

ELISA

1:50,000

Immunoprecipitation

1:200

Western Blot

1:2,500-1:5,000

Protein

Uniprot #

O75369

Overview

Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton.

Molecular Function

actin binding

cadherin binding

identical protein binding

RNA binding

Subcellular Location

Cytoskeleton

Expression

Ubiquitous. Isoform 1 and isoform 2 are expressed in placenta, bone marrow, brain, umbilical vein endothelial cells (HUVEC), retina and skeletal muscle. Isoform 1 is predominantly expressed in prostate, uterus, liver, thyroid, stomach, lymph node, small intestine, spleen, skeletal muscle, kidney, placenta, pancreas, heart, lung, platelets, endothelial cells, megakaryocytic and erythroleukemic cell lines. Isoform 2 is predominantly expressed in spinal cord, platelet and Daudi cells. Also expressed in thyroid adenoma, neurofibrillary tangles (NFT), senile plaques in the hippocampus and cerebral cortex in Alzheimer disease (AD). Isoform 3 and isoform 6 are expressed predominantly in lung, heart, skeletal muscle, testis, spleen, thymus and leukocytes. Isoform 4 and isoform 5 are expressed in heart.

Structure

Homodimer. Interacts with MICALL2. Interacts with RFLNA and RFLNB. Isoform 1 interacts with FBLP1, FLNA, FLNC, GP1BA, INPPL1, ITGB1A, PSEN1 and PSEN2. Isoform 3 interacts with ITGB1A, ITGB1D, ITGB3 and ITGB6.

Alternative Nomenclature

ATPDase antibody

CD 39 antibody

Ecto apyrase antibody

Ecto ATP diphosphohydrolase antibody

Ecto-apyrase antibody

Ecto-ATP diphosphohydrolase 1 antibody

Ecto-ATPase 1 antibody

Ecto-ATPDase 1 antibody

Ectonucleoside triphosphate diphosphohydrolase 1 antibody

ENTPD 1 antibody

Lymphoid cell activation antigen antibody

NTPDase 1 antibody

SPG64 antibody