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主营:hosphodieterase抗体,磷酸二酯酶及四维抗体,葡萄糖转运蛋白抗体和多巴胺受体抗体
℡ 4000-520-616
℡ 4000-520-616
FabGennix/StripOBuffer Western Blot Stripping Buffer/FGI-1989/
产品编号:FGI-1989
市  场 价:¥3000.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>功能性抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$150.00
品      牌: FabGennix
公      司:FabGennix
公司分类:
FabGennix/StripOBuffer Western Blot Stripping Buffer/FGI-1989/
商品介绍

Overview

 

 

Product

 

StripOBufferTM

Description

StripOBufferTMisaspeciallyformulatedwaterbasedbufferthatwillstripantibodiesfromantigensin5-10minutesatroomtemperature

Volume

100ML

Concentration

10Xsolution

Storage

Roomtemperatureforshort-termstorage

Applications

25mLofStripObuffercanbeusedon25-50mini-blots.

 

Advantages

 

 

  • Savetimebyre-usingblots
  • Preserveproteinandantigensamples
  • EliminateissueswithproperequalproteinloADIng
  • Comparephosphorylatedandnon-phosphorylatedstateofthesameprotein
    onthesameblot
  • Reconfirmproteinexpressionbyre-probingthesameblotwithdifferentsetof
    antibodiesraisedagainstantibodiesfromdifferentvendorsorsameordifferentepitopes
  • Preformmultiplestripping’swhilemaintainingantigenicity
  • Noheatingrequired
  • Noβ-mercaptoethanolrequired–Nosmell
  • CompatIBLewithNitrocelluloseandPVDFmembranes

Protocol

 

 

  1. Dilute(1:10)thesuppliedbufferindistilledwaterpriortouse.
  2. Rinsethemembraneindistilledwaterfirstandtheninstrippingbufferatleastonce. 
    Multiplerinseswithbufferresultsinclearerbandsonautoradiograms
  3. Immersethemembranecompletelyin10mLofstrippingbufferforeach
    mini-gelmembrane.
  4. Gentlyshakethemembraneonarockerfor5-10minutesatroomtemperature.
  5. Rinsethemembranewithdistilledwater.
  6. RinsethemembranewithTBSTorthebufferinwhichyourprimaryantibodyisdiluted.
  7. Proceedforblockingstage.

 

 

品牌介绍
Fabgennix公司是一家知名的抗体研发生产企业,为全球生物技术公司、科研单位和生物制药公司提供一抗、二抗、试剂、试剂盒、多肽和阳性质控品等产品。 基于坚实的科研背景,Fabgennix公司已成为全球免疫诊断市场高质量单克隆抗体的优质供应商。
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