Affinity PurifiedPost-GPI attachment to proteins 3 Antibody Mid-Region
Verified Applications
DB, ELISA, IHC, IP, WB
Host
Rabbit
Species Cross Reactivity
Human, Monkey
Immunogen
Synthetic peptide taken within amino acid region 10-60 on human PGAP3 protein.
Accession #
GenBank: BC010652
Physical Properties
Quantity
100 µg
Volume
200 µl
Concentration
0.50-0.75 µg/µl in antibody stabilization buffer
Form
Affinity Purified
Clonality
Polyclonal
Determinant
Mid-Region
Storage
-20⁰C for long term storage
Application Protocol
DB
1:50,000
ELISA
1:50,000
Immunohistochemistry
1:100
Immunoprecipitation
1:150
Western Blot
1:500-1:1,000
Protein
Uniprot #
Q96FM1
Overview
Involved in lipid remodeling steps of GPI-anchor maturation. Remodeling steps consist in the generation of 2 saturated fatty chains at the sn-2 position of GPI-anchor proteins.
Molecular Function
Hydrolase activity, acting on ester bonds, GPI-anchor biosynthesis
Subcellular Location
Golgi apparatus; Endoplasmic reticulum; Membrane
Expression
Ubiqutously expressed; highest levels in thyroid in placenta