General Information

Product

Helicobacter pylori Antibody

Verified Applications

CM, ELISA, ICC, IF, IP, IHC, WB

Host

Rabbit

Species Cross Reactivity

Helicobacter pylori

Immunogen

Heat killed and denatured Helicobacter pylori extracts

Physical Properties

Quantity

100 µg

Volume

200 µl

Concentration

0.80-1.35 µg/µl in antibody stabilization buffer

Form

Affinity Purified

Clonality

Polyclonal

Storage

-20⁰C for long term storage

Application Protocol

Confocal Microscopy

1:200

ELISA

1:5,000

Immunocytochemistry

1:200

Immunofluorescence

1:300

Immunohistochemistry

1:200

Immunoprecipitation

1:150

Western Blot

1:500

Protein

Uniprot #

Q96CV9

Overview

Plays an important role in the maintenance of the Golgi complex, in membrane trafficking, in exocytosis, through its interaction with myosin VI and Rab8. Links myosin VI to the Golgi complex and plays an important role in Golgi ribbon formation. Negatively regulates the induction of IFNB in response to RNA virus infection.

Molecular Function

polyubiquitin binding, Rab GTPase binding, protein C-terminus binding

Subcellular Location

Cytoplasm › perinuclear region. Golgi apparatus. Golgi apparatus › trans-Golgi network. Cytoplasmic vesicle ›autophagosome. Cytoplasmic vesicle. Recycling endosome

Expression

Present in aqueous humor of the eye (at protein level). Highly expressed in trabecular meshwork. Expressed nonpigmented ciliary epithelium, retina, brain, adrenal cortex, fetus, lymphocyte, fibroblast, skeletal muscle, heart, liver, brain and placenta.

Structure

Interacts with HD, Rab8 (RAB8A and/or RAB8B) (active GTP-bound form), GTF3A, TRAF3, TBK1, MYO6 and TFRC. Binds to linear ubiquitin chains. Interacts with LC3 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2; OPTN phosphorylation increas

Alternative Nomenclature

H. pylori antibody